Composite

Part:BBa_K5115060:Design

Designed by: Liyue Chen   Group: iGEM24_Fudan   (2024-09-19)


ribozyme+RBS+cso(without csoS3)+stem-loop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 208
    Illegal NotI site found at 5126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1825
    Illegal AgeI site found at 2506
    Illegal AgeI site found at 3460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 266


Design Notes

none


Source

synthetic

References